{"id":9294,"date":"2026-06-29T10:30:55","date_gmt":"2026-06-29T10:30:55","guid":{"rendered":"https:\/\/mexipedium.ch\/?page_id=9294"},"modified":"2026-06-29T15:37:31","modified_gmt":"2026-06-29T15:37:31","slug":"aussaat","status":"publish","type":"page","link":"https:\/\/mexipedium.ch\/en\/aussaat\/","title":{"rendered":"Aussaat"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"9294\" class=\"elementor elementor-9294\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dfdc5a6 e-flex e-con-boxed wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-parent\" data-id=\"dfdc5a6\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-1eaf493 e-con-full e-flex wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-child\" data-id=\"1eaf493\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2ea6a7d elementor-widget elementor-widget-heading\" data-id=\"2ea6a7d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Sowing Mexipedium Seed<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-bf40575 e-flex e-con-boxed wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-parent\" data-id=\"bf40575\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-309a961 e-con-full e-flex wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-child\" data-id=\"309a961\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-58bc569 elementor-widget elementor-widget-text-editor\" data-id=\"58bc569\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>1. Introduction<\/h3><p class=\"translation-block\">Asymbiotic sowing is an artificial germination method for orchid seeds that does not rely on the natural symbiosis with mycorrhizal fungi. While orchid seeds have extremely low germination rates in nature, sterile culture on nutrient media enables controlled, successful germination. <br>This method is used by both professional laboratories and hobbyists to propagate rare or sensitive species. The cultivation of <i>Mexipedium xerophyticum<\/i> is considered quite challenging and is practiced by only a very small number of growers. It has also been reported that seed production alone is a challenge. We have not had this experience so far, although empty capsules are regularly observed.<\/p><p class=\"translation-block\">The process described here explains how to sow <i>Mexipedium xerophyticum<\/i> from a green, unopened capsule. Whenever possible, this method is preferable to sowing seeds from a dry, opened capsule.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b17bfa8 elementor-widget elementor-widget-image\" data-id=\"b17bfa8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1800\" height=\"995\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995.jpg\" class=\"attachment-full size-full wp-image-9331\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995.jpg 1800w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/kapseln_1800_995-18x10.jpg 18w\" sizes=\"(max-width: 1800px) 100vw, 1800px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text translation-block\">Seed capsules of <i>Mexipedium xerophyticum<\/i>. It is already apparent that two of the capsules likely do not contain viable seeds.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7c964ee elementor-widget elementor-widget-text-editor\" data-id=\"7c964ee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>2. Materials Needed<\/h3><p>\u00a0Before you begin, make sure you have all the materials ready.<\/p><h4>2.1. Equipment<\/h4><ul><li style=\"font-size: 16px;\">Sterile workbench with a HEPA filter (alternatives are available for hobbyists)<\/li><li>Autoclave or pressure cooker for sterilization<\/li><li>Glass jars, screw-top containers, or culture bottles (e.g., baby food jars)<\/li><li>Tweezers, scalpel, spoon, and spatula<\/li><li>Spray bottle containing alcohol (70% ethanol or isopropanol)<\/li><li>Precision scale<\/li><li>pH meter or pH test strips (the former is definitely preferable)<\/li><li>Measuring cup<\/li><li>A pot and stove for preparing the culture medium<\/li><li>Glass bead or infrared sterilizer<\/li><li>Laboratory tool rack<\/li><li>Roll of plastic film for sealing the culture containers<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9c55fd9 elementor-widget elementor-widget-image\" data-id=\"9c55fd9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1800\" height=\"995\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995.jpg\" class=\"attachment-full size-full wp-image-9332\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995.jpg 1800w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2025\/12\/materialien_1800_995-18x10.jpg 18w\" sizes=\"(max-width: 1800px) 100vw, 1800px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Materials and equipment for sowing orchid seeds<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4917c1c elementor-widget elementor-widget-text-editor\" data-id=\"4917c1c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4>2.2. Chemicals<\/h4><p><span style=\"font-style: inherit; font-weight: inherit;\"><br \/>The following chemicals are needed during the sowing process:<\/span><\/p><ul><li>Disinfectant for surface sterilization of the seed capsule (e.g., 10% household bleach with a drop of dish soap)<\/li><li>70% ethyl alcohol (ethanol) for surface disinfection<\/li><li>NaOH (caustic soda) to raise the pH level<\/li><li>HCl (hydrochloric acid) for lowering the pH<\/li><li>Murashige &amp; Skoog (MS) Medium, powder for preparing growing medium for orchid seed sowing<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-842646a elementor-widget elementor-widget-text-editor\" data-id=\"842646a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4>2.3. Culture Medium<\/h4><p class=\"translation-block\">After countless experiments with various culture media and organic additives, it has become clear that  <b>coconut water<\/b> is an essential component for the successful growth of <em>Mexipedium xerophyticum<\/em>.  Since we began breeding <em>Mexipedium<\/em>, we have varied the coconut water concentration and have concluded that optimal growth is achieved at 10%. This concentration has proven effective for both sowing and replating. We have also conducted experiments with concentrations of up to 30% by volume of the medium. However, after an initially good germination rate, the protocorms soon die off.<\/p><p class=\"translation-block\">In general, it is easy to get <i>Mexipedium xerophyticum<\/i> to germinate. However, further growth is difficult and can only be achieved on perfectly formulated culture media.<\/p><p class=\"translation-block\">In addition to coconut water, we also conducted experiments with <b>pineapple juice<\/b> and <b>homogenized banana<\/b> (see photo below). We also varied the sugar concentration. It turned out that all organic additives (with the exception of coconut water) had a negative effect on the growth of the young plants, regardless of their concentration. They had no effect on germination itself though.<\/p><p class=\"translation-block\">For <i>Mexipedium xerophyticum<\/i>, we have found the following growing medium to be effective:<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-988b895 eael-table-align-center eael-dt-th-align-left elementor-widget elementor-widget-eael-data-table\" data-id=\"988b895\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-data-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"eael-data-table-wrap\" data-table_id=\"988b895\" id=\"eael-data-table-wrapper-988b895\" data-custom_responsive=\"false\">\n\t\t\t<table class=\"tablesorter eael-data-table center\" id=\"eael-data-table-988b895\">\n\t\t\t    <thead>\n\t\t\t        <tr class=\"table-header\">\n\t\t\t\t\t\t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Composition of 1000 ml of sowing medium<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Quantity<\/span><\/th>\n\t\t\t        \t\t\t\t        <\/tr>\n\t\t\t    <\/thead>\n\t\t\t  \t<tbody>\n\t\t\t\t\t\t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tAgar-Agar\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t7g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tMS Medium (1\/2 concentration)\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t2.3g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tCoconut water\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t100ml\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tSucrose\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t12g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tActivated charcoal\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t2g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tDistilled water\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t900ml\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tHCl \/ NaOH\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tpH: 5.8 &#8211; 6.0\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t    <\/tbody>\n\t\t\t<\/table>\n\t\t<\/div>\n\t  \t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a5da3a2 elementor-widget elementor-widget-image\" data-id=\"a5da3a2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"2560\" height=\"1415\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-scaled.jpg\" class=\"attachment-full size-full wp-image-9401\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-scaled.jpg 2560w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-2048x1132.jpg 2048w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/ms_medium-18x10.jpg 18w\" sizes=\"(max-width: 2560px) 100vw, 2560px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">MS medium (Murashige &amp; Skoog) is one of the best-known culture media for orchid propagation.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-af6bea2 elementor-widget elementor-widget-image\" data-id=\"af6bea2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1415\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-scaled.jpg\" class=\"attachment-full size-full wp-image-9402\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-scaled.jpg 2560w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-2048x1132.jpg 2048w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro_vergleich-18x10.jpg 18w\" sizes=\"(max-width: 2560px) 100vw, 2560px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Experiment: 6-month-old seedlings; on the left, grown in a 1\/2 MS solution with 10% coconut water and 5% pineapple juice. On the right, the same mixture without pineapple juice.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-41e3942 elementor-widget elementor-widget-text-editor\" data-id=\"41e3942\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0<\/p><h3>3. Preparation of the culture medium<\/h3><p>The growth medium serves as the seedlings' sole source of nutrients. We will prepare the culture containers as follows:<\/p><ol><li>Dissolve the agar in a beaker with some of the available distilled water. It will dissolve more easily in the hot media if it has already been dissolved in cold liquid beforehand.<\/li><li>Pour the remaining liquids into the pot and bring to a boil.<\/li><li>Add MS Medium.<\/li><li>Add agar-agar.<\/li><li><span style=\"font-style: inherit; font-weight: inherit;\">Adjust the pH: Target: pH 5.8\u20136.0. (HCl lowers the pH; NaOH raises it.) Keep in mind that coconut water and pineapple juice naturally contain a lot of acid, which is why adjusting the pH is very important.<\/span><\/li><li>Pour the hot medium into sterilizable containers (glass, polypropylene, or similar).<\/li><li>Loosely seal the container, then sterilize it in an autoclave or pressure cooker at 121 \u00b0C for 20\u201325 minutes.<\/li><li>After sterilizing, seal immediately and let cool.<\/li><li>Seal the gap between the lid and the container with plastic wrap (e.g., cling film).<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9b4a45e elementor-widget elementor-widget-text-editor\" data-id=\"9b4a45e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>4. Preparing the seed pod<\/h3>\n\nVerwende nur reife, aber unbesch\u00e4digte Samenkapseln. Der beste Zeitpunkt ist kurz bevor die Kapsel nat\u00fcrlich aufspringt. Bei <i>Mexipedium xerophyticum<\/i> sind die Kapseln nach ca. 6 Monaten reif. Die restliche Zeit ben\u00f6tigen sie lediglich, um zu trocknen und aufzuplatzen.\n\nWenn immer m\u00f6glich ist die Aussaat von Samen aus der gr\u00fcnen Kapsel derjenigen aus der aufgeplatzten Kapsel vorzuziehen. Das Vorgehen zur Sterilisation der Samenkapsel ist wie folgt:\n\n<ol>\n  <li>Kapsel in Bleichel\u00f6sung (10 % mit 1 Tropfen Sp\u00fclmittel) 15 Minuten einlegen und regelm\u00e4ssig sch\u00fctteln.<\/li>\n  <li>Kapsel gr\u00fcndlich mit sterilem, destilliertem Wasser absp\u00fclen.<\/li>\n  <li>Unter sterilen Bedingungen aufschneiden und die Samen direkt entnehmen.<\/li>\n<\/ol>\n\nBemerkungen: Natriumhypochlorit (NaOCl) wird in verschiedenen Konzentrationen verkauft, wobei Haushaltsbleiche typischerweise 5\u20136 % enth\u00e4lt. Eine 10%ige L\u00f6sung davon bedeutet somit, dass in der fertigen Bleichel\u00f6sung f\u00fcr die Samen- und Kapseldesinfektion von Orchideen ca. 0,5\u20130,6 % NaOCl enthalten sind. Mit dieser Konzentration arbeiten auch viele Labore.\n\nIm Labor kommt anstatt Sp\u00fclmittel meist Tween 20 in einer Konzentration von 1 Tropfen auf 100 ml Bleichel\u00f6sung zum Einsatz. Sp\u00fclmittel in derselben Konzentration funktioniert jedoch genauso gut.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0abc1c2 elementor-widget elementor-widget-text-editor\" data-id=\"0abc1c2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t&nbsp;\n<h3>5. Vorbereitung der sterilen Werkbank<\/h3>\nDie gr\u00f6\u00dfte Gefahr bei der In-vitro-Aussaat sind Schimmelpilze und Bakterien. Um steril arbeiten zu k\u00f6nnen, kommt man fr\u00fcher oder sp\u00e4ter nicht um die Verwendung einer Sterilen Werkbank herum. Dieses Laborger\u00e4t wurde speziell f\u00fcr diese Art von Arbeiten konzipiert. Wir verwenden f\u00fcr unsere Arbeiten eine Sterile Werkbank, die zus\u00e4tzlich mit UV-Licht ausgestattet ist. Die Vorbereitungsarbeit l\u00e4uft wie folgt ab:\n<ol>\n \t<li>UV-Lampe einschalten (typischerweise 15\u201330 Minuten, gem\u00e4ss Herstellerangaben).\u00a0W\u00e4hrenddessen keine Arbeiten durchf\u00fchren.<\/li>\n \t<li>UV ausschalten, Werkbank \u00f6ffnen.<\/li>\n \t<li>Luftstrom aktivieren.<\/li>\n \t<li>10 Minuten vorlaufen lassen, damit Partikel aus dem Arbeitsbereich gesp\u00fclt werden.<\/li>\n \t<li>Arbeitsfl\u00e4che, Seitenw\u00e4nde und Scheibe mit 70 % Ethanol oder Isopropanol abwischen.\u00a0Von oben nach unten und von hinten nach vorne wischen. Saubere, fusselfreie T\u00fccher verwenden.<\/li>\n \t<li>Materialien einbringen.\u00a0Nur notwendige, saubere\/sterile Materialien in die Werkbank stellen. Die Oberfl\u00e4chen s\u00e4mtlicher Materialien sind vor dem Einbringen zu desinfizieren. Daf\u00fcr wird in der Regel ebenfalls Ethanol bzw. Isopropanol verwendet.<\/li>\n \t<li>Nach 20 Minuten ist die Sterile Werkbank vorbereitet und es kann mit der Arbeit begonnen werden.<\/li>\n<\/ol>\nHinweis: UV ersetzt nicht die chemische Desinfektion; sie ist eine zus\u00e4tzliche Massnahme.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-37c44e0 elementor-widget elementor-widget-text-editor\" data-id=\"37c44e0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>6. Seeding onto the culture medium<\/h3><p>Once the seeds and jars are sterile, you can begin sowing:<\/p><ol><li>Wipe the glass surface with ethanol or isopropanol.<\/li><li>From now on, all instruments will be heated (using a ball sterilizer or infrared sterilizer) before coming into contact with the organic material.<\/li><li>Use tweezers to place the seed capsule on a glass slide, then cut it open with a scalpel.<\/li><li>Open the seed tray and set the lid aside with the opening facing up.<\/li><li>Spread the seeds thinly and evenly over the surface of the agar.<\/li><li>Close the lid immediately.<\/li><\/ol><p class=\"translation-block\">Important: Seeds must <b>not be pressed<\/b> into the growing medium; they should simply rest on top of it.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8bda1bc elementor-widget elementor-widget-image\" data-id=\"8bda1bc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1800\" height=\"995\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995.jpg\" class=\"attachment-full size-full wp-image-9361\" alt=\"Mexipedium xerophyticum Samen\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995.jpg 1800w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/samen_1800_995-18x10.jpg 18w\" sizes=\"(max-width: 1800px) 100vw, 1800px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text translation-block\">The seeds of <i>Mexipedium xerophyticum<\/i> are black and tiny (size comparison with a 1 Swiss franc coin).<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-93a363e elementor-widget elementor-widget-text-editor\" data-id=\"93a363e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>6.1. Nach der Aussaat<\/h3>\nSind s\u00e4mtliche Aussaaten erledigt, werden die Gef\u00e4sse noch in der Werkbank mit Kunststoffolie abgedichtet und ggf. beschriftet, falls dies nicht schon geschehen ist. Dabei ist nicht nur die Information zur Kreuzung, sondern auch das Aussaatdatum von grosser Wichtigkeit.\n\nDie Gl\u00e4ser werden an einem warmen Ort bei 22\u201324 \u00b0C aufgestellt. Die Temperatur sollte dabei so wenig schwanken wie m\u00f6glich. Wir haben die Erfahrung gemacht, dass die Protokorme bei <i>Mexipedium xerophyticum<\/i> gr\u00f6sser werden, wenn die Keimung im Dunkeln erfolgt. Der Unterschied zu im Licht gekeimten S\u00e4mlingen ist aber fast vernachl\u00e4ssigbar, weshalb wir sie normalerweise von Anfang an zu den anderen Aussaatgef\u00e4ssen stellen.\n\nBesonders wichtig ist an der Stelle zu erw\u00e4hnen, dass die Protokorme und Jungpflanzen sehr anf\u00e4llig f\u00fcr zu hohe Temperaturen sind. Es ist deshalb essenziell, dass das Thermometer m\u00f6glichst zu keiner Zeit \u00fcber 28 \u00b0C ansteigt. Spezialisierte Orchideenlabore haben in der Vergangenheit schon ihre gesamten Aussaatgef\u00e4sse mit <i>Mexipedium xerophyticum<\/i> aufgrund von zu hohen Temperaturen verloren!\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a9e703 elementor-widget elementor-widget-text-editor\" data-id=\"6a9e703\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>7. Germination and Care of the Protocorms<\/h3><p class=\"translation-block\">The first seedlings appear after about <strong>2 weeks<\/strong>.<\/p><h4>7.1. Development Phases<\/h4><div>\u00a0<\/div><div><ol style=\"list-style-type: upper-latin;\"><li>Swelling phase: The embryo absorbs moisture. (Starting on Day 1)<\/li><li>Rupture of the testa: The embryo swells so much that it ruptures the testa (seed coat). (Approx. 5\u201310 days after sowing)<\/li><li>Protocorm formation: The embryo develops into an undifferentiated ball of cells, known as the protocorm. (Approx. 10\u201315 days after sowing)<\/li><li>Differentiation phase: The seedling develops leaf primordia and absorbs nutrients and water with the help of rhizoids (the precursors to roots). (Approx. 2 months after sowing)<\/li><li>Root formation: The first root tips appear and anchor the seedling in the growing medium. (Approx. 5 months after sowing)<\/li><li>Growth phase: The young plant continues to develop, and new roots and leaves are constantly forming. (Ready for transplanting after about 2 years)<\/li><\/ol><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ab3ab69 elementor-widget elementor-widget-image\" data-id=\"ab3ab69\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1204\" height=\"329\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/mexipedium_germination.jpg\" class=\"attachment-full size-full wp-image-9387\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/mexipedium_germination.jpg 1204w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/mexipedium_germination-300x82.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/mexipedium_germination-1024x280.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/mexipedium_germination-768x210.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/mexipedium_germination-18x5.jpg 18w\" sizes=\"(max-width: 1204px) 100vw, 1204px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text translation-block\">Schematic representation of the developmental stages of <i>Mexipedium xerophyticum<\/i> from seed to a young plant ready for transplanting.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6f7c12d elementor-widget elementor-widget-text-editor\" data-id=\"6f7c12d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4>7.2. Growth Conditions for Protocorms<\/h4>\n<span style=\"font-size: 1rem;\">Wie schon oben beschrieben k\u00f6nnen die Kulturgef\u00e4sse von Anfang an ans Licht gestellt werden, weil kaum Unterschiede zu im Dunkeln gekeimten S\u00e4mlingen zu erkennen sind.<\/span>\n\nFalls man sich dennoch f\u00fcr die Dunkelkeimung entscheidet,\u00a0werden die Gef\u00e4sse ans Licht gestellt, nachdem sich die Samen mehrheitlich in Protokorme verwandelt haben. Dies geschieht nach ungef\u00e4hr 6 Wochen. Schnell setzt die Chlorophyllbildung ein und die Protokorme werden innert Wochenfrist gr\u00fcn.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-92b9fba elementor-widget elementor-widget-image\" data-id=\"92b9fba\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1415\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-scaled.jpg\" class=\"attachment-full size-full wp-image-9368\" alt=\"Mexipedium xerophyticum Keimlinge\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-scaled.jpg 2560w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-2048x1132.jpg 2048w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/01\/keimung_12d_1800_995-18x10.jpg 18w\" sizes=\"(max-width: 2560px) 100vw, 2560px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Just 12 days after sowing, you can see how the testa (the seed coat made up of dead cells) splits open and the embryo swells.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5d64059 elementor-widget elementor-widget-image\" data-id=\"5d64059\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2000\" height=\"1000\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme.jpg\" class=\"attachment-full size-full wp-image-9394\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme.jpg 2000w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme-300x150.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme-1024x512.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme-768x384.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme-1536x768.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/02\/protokorme-18x9.jpg 18w\" sizes=\"(max-width: 2000px) 100vw, 2000px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">After 45 days, the protocorms have turned green and are showing the first signs of leaf buds.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-93e5ad1 elementor-widget elementor-widget-text-editor\" data-id=\"93e5ad1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4>7.3. When to replate?<\/h4><p>After about 4\u20135 months, the seedlings are replated for the first time. By this point, the seedlings will have already begun to form their first leaves. Replating them into a new growing medium has the advantage of allowing for greater spacing between the seedlings, which improves their access to nutrients and light.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6b5d7a3 elementor-widget elementor-widget-text-editor\" data-id=\"6b5d7a3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>8. Replating onto fresh medium<\/h3><p class=\"translation-block\">In our experience, when transplanting <i>Mexipedium xerophyticum<\/i>, it is best to use the same growing medium as was used for sowing. Unlike with other lady\u2019s slipper species, homogenized banana had a negative effect on the development of the young plants at all concentrations. Other organic additives, such as pineapple juice, also had no positive effect on the development of the young plants or even inhibited it.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-14bfabd eael-table-align-center eael-dt-th-align-left elementor-widget elementor-widget-eael-data-table\" data-id=\"14bfabd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-data-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"eael-data-table-wrap\" data-table_id=\"14bfabd\" id=\"eael-data-table-wrapper-14bfabd\" data-custom_responsive=\"false\">\n\t\t\t<table class=\"tablesorter eael-data-table center\" id=\"eael-data-table-14bfabd\">\n\t\t\t    <thead>\n\t\t\t        <tr class=\"table-header\">\n\t\t\t\t\t\t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Composition of 1000 ml of sowing medium<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Quantity<\/span><\/th>\n\t\t\t        \t\t\t\t        <\/tr>\n\t\t\t    <\/thead>\n\t\t\t  \t<tbody>\n\t\t\t\t\t\t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tAgar-Agar\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t7g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tMS Medium (1\/2 concentration)\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t2.3g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tCoconut water\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t100ml\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tSucrose\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t12g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tActivated charcoal\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t2g\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tDistilled water\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t900ml\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tHCl \/ NaOH\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\tpH: 5.8 &#8211; 6.0\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t    <\/tbody>\n\t\t\t<\/table>\n\t\t<\/div>\n\t  \t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4694f77 elementor-widget elementor-widget-image\" data-id=\"4694f77\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1415\" src=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-scaled.jpg\" class=\"attachment-full size-full wp-image-9403\" alt=\"\" srcset=\"https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-scaled.jpg 2560w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-300x166.jpg 300w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-1024x566.jpg 1024w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-768x425.jpg 768w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-1536x849.jpg 1536w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-2048x1132.jpg 2048w, https:\/\/mexipedium.ch\/wp-content\/uploads\/2026\/06\/in_vitro-18x10.jpg 18w\" sizes=\"(max-width: 2560px) 100vw, 2560px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">After 5 months, these young plants were transplanted for the first time. This photo was taken one month later.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-219d77e elementor-widget elementor-widget-text-editor\" data-id=\"219d77e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4>8.1. The Replating Process<\/h4><ol><li>Work under sterile conditions again.<\/li><li>Carefully remove the young plants from the jar using a sterile spatula or, later on, a pair of long tweezers. We use Dennerle XL Plant Tweezers for this because they have a high-quality metal finish and provide a comfortable amount of resistance when squeezing.<\/li><li>Place it in fresh growing medium, leaving enough space around it. If there are roots, try to bring them into contact with the growing medium as much as possible.<\/li><li>Close the jar again, seal it with plastic wrap, and continue growing.<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-de72629 elementor-widget elementor-widget-text-editor\" data-id=\"de72629\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>9. Potting the Plants<\/h3><p>After about two years, the plants are big enough to be removed from their growing containers and planted in a community pot.<\/p><h4>9.1. Preparing the Culture Containers<\/h4><p>The choice of plastic containers is a matter of personal preference. What's much more important is the potting mix:<\/p><p class=\"translation-block\"><b>Fine pine bark + perlite + a little <i>Sphagnum<\/i>.<\/b><\/p><p class=\"translation-block\">Tip: A thin layer of <i>Sphagnum<\/i> should be spread over the soil surface to keep the base of the young plants moist.<\/p><h4>9.2. Step-by-Step Procedure<\/h4><div>\u00a0<\/div><ol><li>Remove the plants from the jar and thoroughly rinse off any remaining agar under running water. Take care not to damage the roots. It is always better to leave a little medium on the roots than to try to clean the plants too thoroughly.<\/li><li>Put it in the community pot.<\/li><li class=\"translation-block\">Apply a thin layer of <i>Sphagnum<\/i> to the surface of the substrate after transplanting all the seedlings.<\/li><li>Maintain high humidity levels during the first few weeks. Air circulation is extremely important during this time to prevent disease.<\/li><li>Once the plants are sufficiently established, they can tolerate lower humidity.<\/li><\/ol><div class=\"translation-block\">Note: During this phase, the young plants of <i>Mexipedium xerophyticum<\/i> grow exceptionally slowly compared to other lady's slipper orchids. This is normal.<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a53b951 elementor-widget elementor-widget-text-editor\" data-id=\"a53b951\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>10. Common Problems and Solutions<\/h3>\n<div><\/div>\n<h4>10.1. Mold in the jar<\/h4>\n<p class=\"translation-block\"><strong>Cause:<\/strong> Insufficient sterility.<br><strong>Solution:<\/strong> Discard the glass; conduct a root cause analysis.<\/p><p><span style=\"color: rgb(255, 255, 255); font-family: Montserrat, sans-serif; font-size: 1.52941rem; font-style: inherit; font-weight: 600;\">10.2. A cloudy film on the surface of the medium<\/span><\/p>\n<p class=\"translation-block\"><strong>Cause:<\/strong> Bacterial growth: Insufficient sterility.<br><strong>Solution:<\/strong> Discard the glass; conduct a root cause analysis.<br>Alternative: Given the rarity of the species, you can try to save the young plants. This actually worked quite well in our own experiments. To do this, immerse the young plants in a disinfectant solution (10% household bleach with a drop of dish soap) for at least 30 minutes, shaking them regularly. Afterward, the plants can be transferred to a new medium (without rinsing them with sterile distilled water). This treatment is not particularly gentle on the plants, and some will die in the process, but a surprisingly large proportion survives this procedure. Because the disinfectant remains on the young plants, it provides long-lasting protection against all types of germs that may still be present on the contaminated plant parts.<\/p><p><span style=\"color: rgb(255, 255, 255); font-family: Montserrat, sans-serif; font-size: 1.52941rem; font-style: inherit; font-weight: 600;\">10.3. No Germination<\/span><\/p>\n<div>\n<strong>Ursache M\u00f6glichkeit 1:<\/strong> Samen m\u00f6glicherweise tot bzw. nicht fertil.<br><strong>L\u00f6sung:<\/strong> Abwarten und Glas nach 1 Jahr entsorgen. Die Wahrscheinlichkeit, dass dann noch etwas keimt, geht gegen null.<p><\/p>\n<p><strong>Ursache M\u00f6glichkeit 2:<\/strong> pH-Wert fehlerhaft.<br><strong>L\u00f6sung:<\/strong> pH-Wert genau feststellen, bevor weitergefahren wird.<\/p>\n<p><strong>Ursache M\u00f6glichkeit 3:<\/strong> Unpassende Wachstumsbedingungen.<br><strong>L\u00f6sung:<\/strong> Wachstumsbedingungen genau \u00fcberwachen. Die Temperatur ist besonders wichtig (Minimum-Maximum-Thermometer).<\/p><p><span style=\"color: rgb(255, 255, 255); font-family: Montserrat, sans-serif; font-size: 1.52941rem; font-style: inherit; font-weight: 600;\">10.4. Wachstumsstillstand<\/span><\/p><\/div>\n<div>\n<strong>Ursache:<\/strong> Medium verbraucht.<br><strong>L\u00f6sung:<\/strong> Umlegen notwendig.<p><\/p>\n<p><strong>Ursache:<\/strong> Unpassende Wachstumsbedingungen.<br><strong>L\u00f6sung:<\/strong> Wachstumsbedingungen genau \u00fcberwachen. Besonders kritisch sind bei <i>Mexipedium xerophyticum<\/i> zu hohe Temperaturen. Diese sollten 28 \u00b0C m\u00f6glichst nie \u00fcbersteigen, auch nicht kurzfristig!<\/p><p><span style=\"color: rgb(255, 255, 255); font-family: Montserrat, sans-serif; font-size: 1.52941rem; font-style: inherit; font-weight: 600;\">10.5. Ausbleichen der Protokorme<\/span><\/p><\/div>\n<div>\n<p><strong>Ursache 1:<\/strong> Falsches Medium.<br><strong>L\u00f6sung:<\/strong> Oben beschriebenes Medium verwenden. <i>Mexipedium xerophyticum<\/i> ist sehr w\u00e4hlerisch, was das Medium und vor allem die organischen Zus\u00e4tze angeht. Die Samen zum Keimen zu bringen ist zwar in der Regel sehr einfach, sie danach aber zum Wachsen zu bringen, stellt eine Herausforderung dar.<\/p>\n<p><strong>Ursache 2:<\/strong> Falsche Temperatur.<br><strong>L\u00f6sung:<\/strong> Die Keimlinge im Glas sind verh\u00e4ltnism\u00e4ssig empfindlich auf zu hohe Temperaturen. Temperaturspitzen \u00fcber 30 \u00b0C quittiert <i>Mexipedium xerophyticum<\/i> mit dem Absterben einzelner Protokorme, bis hin zum Totalverlust.<\/p>\n<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-61b8a0cd e-flex e-con-boxed wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no wpr-column-slider-no wpr-equal-height-no e-con e-parent\" data-id=\"61b8a0cd\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-aee4807 elementor-widget-divider--separator-type-pattern elementor-widget-divider--no-spacing elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"aee4807\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\" style=\"--divider-pattern-url: url(&quot;data:image\/svg+xml,%3Csvg xmlns=&#039;http:\/\/www.w3.org\/2000\/svg&#039; 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Einleitung Die asymbiotische Aussaat ist eine k\u00fcnstliche Keimmethode f\u00fcr Orchideensamen, die ohne die nat\u00fcrliche Symbiose mit Mykorrhizapilzen auskommt. W\u00e4hrend Orchideensamen in der Natur extrem geringe Keimraten haben, erm\u00f6glicht die sterile Kultur auf N\u00e4hrmedien eine kontrollierte, erfolgreiche Keimung.Diese Methode wird sowohl von professionellen Laboren als auch von Hobbyisten genutzt, um seltene oder empfindliche Arten zu vermehren. Die Zucht von Mexipedium xerophyticum gilt dabei als recht anspruchsvoll und wird nur von ganz wenigen Z\u00fcchtern praktiziert. Es wird auch berichtet, dass alleine schon die Samenproduktion eine Herausforderung ist. Diese Erfahrungen machten wir bisher nicht, obschon regelm\u00e4ssig leere Kapseln zu beobachten sind. Der hier beschriebene Prozess beschreibt die Aussaat von Mexipedium xerophyticum aus der gr\u00fcnen, noch unge\u00f6ffneten Kapsel. Wenn immer m\u00f6glich ist diese Methode der Aussaat von Samen aus der trockenen, ge\u00f6ffneten Kapsel vorzuziehen. Samenkapseln von Mexipedium xerophyticum. Schon jetzt erkennt man, dass zwei der Kapseln vermutlich keine fertilen Samen enthalten. 2. Ben\u00f6tigte Materialien \u00a0Bevor du beginnst, stelle sicher, dass du alle Materialien vorbereitet hast. 2.1. Ger\u00e4te Sterile Werkbank mit HEPA-Filter (Alternativen gibt es f\u00fcr den Hobbybereich) Autoklav oder Druckkochtopf zur Sterilisation Gl\u00e4ser, Schraubgef\u00e4sse oder Kulturflaschen (z. B. Babygl\u00e4schen) Pinzette, Skalpell, L\u00f6ffel und Spatel Spr\u00fchflasche mit Alkohol (70% Ethanol oder Isopropanol) Pr\u00e4zisionswaage pH-Meter oder pH-Teststreifen (ersterer ist definitiv vorzuziehen) Messbecher Kochtopf und Herd zur Herstellung des N\u00e4hrmediums Glaskugel- oder Infrarotsterilisator Labor-Werkzeugst\u00e4nder Rolle Kunststofffolie zum Abdichten der Aussaatgef\u00e4sse Materialien und Ger\u00e4te zur Aussaat von Orchideensamen 2.2. Chemikalien Die folgenden Chemikalien werden w\u00e4hrend des Aussaatprozesses ben\u00f6tigt: Desinfektionsmittel zur oberfl\u00e4chlichen Sterilisation der Samenkapsel (z. B. 10%ige Haushaltsbleiche (Javel) mit einem Tropfen Sp\u00fclmittel) Ethylalkohol (Ethanol) 70% zur Fl\u00e4chendesinfektion NaOH (Natronlauge) f\u00fcr die Erh\u00f6hung des pH-Wertes HCl (Salzs\u00e4ure) f\u00fcr die Senkung des pH-Wertes Murashige &amp; Skoog (MS) Medium, Pulver zur N\u00e4hrbodenherstellung f\u00fcr die Orchideenaussaat 2.3. N\u00e4hrmedium Nach unz\u00e4hligen Versuchen mit verschiedenen N\u00e4hrmedien und organischen Zus\u00e4tzen hat sich herausgestellt, dass f\u00fcr das erfolgreiche Wachstum von\u00a0Mexipedium xerophyticum Kokosnusswasser ein essenzieller Bestandteil ist.\u00a0 Seit wir mit Mexipedium z\u00fcchten haben wir die Kokoswasser-Konzentration variiert und sind zum Schluss gekommen, dass ein optimales Wachstum mit 10% erreicht werden kann. Diese Konzentration hat sich sowohl f\u00fcr die Aussaat, als auch f\u00fcr das Umlegen bew\u00e4hrt. Wir haben auch Experimente mit Konzentrationen bis 30% Volumenanteil des Mediums durchgef\u00fchrt. Nach anf\u00e4nglich guter Keimrate sterben die Protokorme jedoch schon bald ab. Generell ist es einfach, Mexipedium xerophyticum zum keimen zu bringen. Das weitere Wachstum ist jedoch schwierig und nur auf perfekt abgestimmten N\u00e4hrmedien erfolgreich. Zus\u00e4tzlich zu Kokosnusswasser haben wir auch Experimente mit Ananassaft und homogenisierter Banane durchgef\u00fchrt (siehe Foto unten). Auch die Zuckerkonzentration haben wir variiert. Es hat sich herausgestellt, dass s\u00e4mtliche organischen Zus\u00e4tze (mit Ausnahme von Kokosnusswasser) einen negativen Effekt auf das Wachstum der Jungpflanzen haben, unabh\u00e4ngig von deren Konzentration. Auf die Keimung selbst hatten sie keinen Einfluss. F\u00fcr Mexipedium xerophyticum hat sich f\u00fcr uns folgender N\u00e4hrboden bew\u00e4hrt: Zusammensetzung 1000ml N\u00e4hrboden (Aussaat) Menge Agar-Agar 7g MS Medium (1\/2 Konzentration) 2.3g Kokoswasser 100ml Saccharose 12g Aktivkohle 2g Destilliertes Wasser 900ml HCl \/ NaOH pH: 5.8 &#8211; 6.0 Das MS-Medium (nach Murashige &amp; Skoog) ist eines der bekanntesten N\u00e4hrmedien f\u00fcr die Orchideenzucht. Experiment: 6 Monate alte Jungpflanzen, links auf 1\/2 MS, 10% Kokoswasser und 5% Ananassaft. Rechts dieselbe Rezeptur ohne Ananassaft. \u00a0 3. Vorbereitung des N\u00e4hrmediums Das N\u00e4hrmedium dient den Keimlingen als alleinige N\u00e4hrstoffquelle. Die Kulturgef\u00e4sse werden wir folgt vorbereitet: Agar in einem Becher mit einem Teil des zur Verf\u00fcgung stehenden destillierten Wassers aufl\u00f6sen. Sp\u00e4ter l\u00f6st er sich besser in der heissen N\u00e4hrl\u00f6sung, wenn er vorg\u00e4ngig schon in kalter Fl\u00fcssigkeit gel\u00f6st wurde. Restliche Fl\u00fcssigkeiten in Gef\u00e4ss geben und aufkochen. MS Medium hinzugeben. Agar-Agar hinzugenben. pH-Wert einstellen: Ziel: pH 5,8\u20136,0. (HCl senkt den pH, NaOH erh\u00f6ht ihn). Zu beachten ist, dass Kokosnusswasser und Ananassaft nat\u00fcrlicherweise viel S\u00e4ure enthalten, weshalb die Korrektur des pH-Wertes sehr wichtig ist. Das heisse Medium in sterilisierbare Beh\u00e4lter f\u00fcllen (Glas, Polypropylen o.\u00e4.). Beh\u00e4lter locker verschliessen, dann 20\u201325 Minuten im Autoklav bzw. Druckkocher bei 121 \u00b0C sterilisieren. Nach dem Sterilisieren schnell verschliessen und abk\u00fchlen lassen. Den Spalt zwischen Deckel und Gef\u00e4ss mit Kunststofffolie (z.B. Frischhaltefolie) abdichten. 4. Vorbereitung der Samenkapsel Verwende nur reife, aber unbesch\u00e4digte Samenkapseln. Der beste Zeitpunkt ist kurz bevor die Kapsel nat\u00fcrlich aufspringt. Bei Mexipedium xerophyticum sind die Kapseln nach ca. 6 Monaten reif. Die restliche Zeit ben\u00f6tigen sie lediglich, um zu trocknen und aufzuplatzen. Wenn immer m\u00f6glich ist die Aussaat von Samen aus der gr\u00fcnen Kapsel derjenigen aus der aufgeplatzten Kapsel vorzuziehen. Das Vorgehen zur Sterilisation der Samenkapsel ist wie folgt: Kapsel in Bleichel\u00f6sung (10 % mit 1 Tropfen Sp\u00fclmittel) 15 Minuten einlegen und regelm\u00e4ssig sch\u00fctteln. Kapsel gr\u00fcndlich mit sterilem, destilliertem Wasser absp\u00fclen. Unter sterilen Bedingungen aufschneiden und die Samen direkt entnehmen. Bemerkungen: Natriumhypochlorit (NaOCl) wird in verschiedenen Konzentrationen verkauft, wobei Haushaltsbleiche typischerweise 5\u20136 % enth\u00e4lt. Eine 10%ige L\u00f6sung davon bedeutet somit, dass in der fertigen Bleichel\u00f6sung f\u00fcr die Samen- und Kapseldesinfektion von Orchideen ca. 0,5\u20130,6 % NaOCl enthalten sind. Mit dieser Konzentration arbeiten auch viele Labore. Im Labor kommt anstatt Sp\u00fclmittel meist Tween 20 in einer Konzentration von 1 Tropfen auf 100 ml Bleichel\u00f6sung zum Einsatz. Sp\u00fclmittel in derselben Konzentration funktioniert jedoch genauso gut.&nbsp; 5. Vorbereitung der sterilen Werkbank Die gr\u00f6\u00dfte Gefahr bei der In-vitro-Aussaat sind Schimmelpilze und Bakterien. Um steril arbeiten zu k\u00f6nnen, kommt man fr\u00fcher oder sp\u00e4ter nicht um die Verwendung einer Sterilen Werkbank herum. Dieses Laborger\u00e4t wurde speziell f\u00fcr diese Art von Arbeiten konzipiert. Wir verwenden f\u00fcr unsere Arbeiten eine Sterile Werkbank, die zus\u00e4tzlich mit UV-Licht ausgestattet ist. Die Vorbereitungsarbeit l\u00e4uft wie folgt ab: UV-Lampe einschalten (typischerweise 15\u201330 Minuten, gem\u00e4ss Herstellerangaben).\u00a0W\u00e4hrenddessen keine Arbeiten durchf\u00fchren. UV ausschalten, Werkbank \u00f6ffnen. Luftstrom aktivieren. 10 Minuten vorlaufen lassen, damit Partikel aus dem Arbeitsbereich gesp\u00fclt werden. Arbeitsfl\u00e4che, Seitenw\u00e4nde und Scheibe mit 70 % Ethanol oder Isopropanol abwischen.\u00a0Von oben nach unten und von hinten nach vorne wischen. Saubere, fusselfreie T\u00fccher verwenden. Materialien einbringen.\u00a0Nur notwendige, saubere\/sterile Materialien in die Werkbank stellen. Die Oberfl\u00e4chen s\u00e4mtlicher Materialien sind vor dem Einbringen zu desinfizieren. Daf\u00fcr wird in der Regel ebenfalls Ethanol bzw. Isopropanol verwendet. Nach 20 Minuten ist die Sterile Werkbank vorbereitet und es kann mit der Arbeit begonnen werden. 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